Our results suggest that the 2D:4D ratio cannot be used as an appropriate trait for the sex determination of human skeletal remains. More than 100 years ago, a difference in the length of the 2 nd, index finger (2D) and the 4 th, ring finger (4D) has been described 1. Analysis of variance revealed that the 2D:4D ratios in our sample varied more by ethnicity than by the sexual identity of the skeletal material. The difference in 2D:4D ratio between the male and female subsamples in both skeletal series was not statistically significant. We have studied 2D:4D ratios obtained from measurements of finger phalanges and metacarpal bones in Russian (45 adult males and 26 adult females) and German (58 adult males and 29 adult females) skeletal series. The aim of our study was to ascertain possibility of using the 2D:4D ratio as an additional marker for sex determination in the study of human skeletal remains. A recent suggestion, that the ratio of the length of the 2nd and 4th digits (2D:4D) is negatively correlated with prenatal testosterone, has led to a number of. Sexual dimorphism in the relative length of the second-to-fourth digits (the digit ratio, or 2D:4D) in humans has been reported in many studies.
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